Workshop on Hydrodynamic and Thermodynamic Analysis of Macromolecules with SEDFIT and SEDPHAT

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Monday, May 20, 2013 - Friday, May 24, 2013
SKU: 20120413
Registration Cutoff Date: 
Monday, April 22, 2013

 

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This workshop reviews strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), and isothermal titration calorimetry (ITC) for the characterization of biological macromolecules in solution.

The first day of the workshop is an introduction into the experimental aspects of AUC, DLS and ITC, which takes place in the laboratory. We also review the basic operation of the instruments; choice of buffers and detection systems; experimental design dependent on the system under study; assembling the AUC cells; and operating the XLA/I. The following days cover many advanced topics of theory, data analysis and experimental setup. These lectures are accompanied by practical computer exercises with the software.

This workshop for researchers with at least limited experience in AUC, DLS and/or ITC. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data.


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Contact

Carline Coote, (301) 496-7975, ccoote@mail.nih.gov