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BIOL 007A | Basic Techniques in Mammalian Cell Culture

October 2, 2019 to October 4, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability.

NIH employees paying with the signed vendor copy of the SF-182 form, please email:

Register Now

Contract: SF-182

This three-day lecture and laboratory course is structured to provide life scientists who are not experienced in mammalian cell culture with an introduction to the principles and practices that will facilitate their ability to develop the use of in vitro systems. After successful completion of the workshop, participants are expected to feel comfortable handling general mammalian cell lines, detecting most common problems in cell culture room and troubleshoot accordingly.

Lecture and Lab Topics
Sterile techniques and cell culture; observing different live mammalian cell lines on inverted phase microscope; cell counting with hemocytometer and automatic cell counter; cell passages; transfection methods and their application; freezing and thawing cells; lipofectamine transfection; fluorescent microscopy; starvation assay and osmotic pressure/cytoskeleton assay; end point dilution and G418 drug selection for producing stable cell lines

Comprehensive Folder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Although no grades are given for Workshops, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 3-day program, each participant will receive 2.1 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% Refund or 100% Full Workshop Credit Available
Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available
Written Notificaiton: 4 to 13 days prior to start of program.
 0% Refund, 100% Full Workshop Credit Available
Written Notificaiton: 3 days or less prior to start of program.
All cancellations must be received in writing via email to Ms. Carline Coote at 
Cancellations received after hours are time marked for the following day.
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation
A special rate is available to all FAES Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility.  Reservations can be made online, see below.

DoubleTree by Hilton Bethesda - Washington DC

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