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BIOL 055 | Engineering with CRISPR, TALENs, and ZFNs

November 4, 2019 to November 8, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: registrar@faes.org or call 301-496-7977 for space availability. 

NIH employees paying with the signed vendor copy of the SF-182 form, please email: registrar@faes.org

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Contract: SF-182

Genomic engineering provides the ability to manipulate gene expression in a variety of desired cell types, including stem cells. This course will provide an overview of the current major genomic engineering platforms such as CRISPR, TALENs, and ZFNs, but it will cover CRISPR genome editing technology in depth.

The first part of the course will focus on the general principles of genome editing protocols, including design, choice of format, delivery, efficiency, specificity, clonal isolation, genotyping, and validation. The second part of the course will address different applications including genome editing in mice, zebrafish, and iPS cells, disease modeling, generation of reporter lines, and high throughput approaches. We will discuss strategies to make CRISPR gene editing more efficient, flexible, and specific. We will explore recent advances in the CRISPR field including base editors and epigenome editing. We will also examine sequencing and quality control considerations for genome editing projects. Hands-on laboratory exercises will accompany the lecture material to provide practical training in design, assembly, transfection, and detection/evaluation steps of a typical genome editing workflow. 

Lecture Topics:
General Principles of Genome Engineering; DNA Repair Mechanisms; CRISPR-mediated Gene Modulation, Epigenome Editing, Targeted Integration, Genome Editing Workflow, Gene Correction in iPSCs; Targeted Mutagenesis in Zebrafish; CRISPR Genome Engineering in ES Cells and Embryos for Mouse Model Generation; Developing Reporter Lines; Sequencing and Quality Control Considerations.

Laboratory Topics:
Introduction to Genome Editing Technologies; CRISPR/Cas9 Design Considerations; Transfection of Cas9/gRNA Ribonucleoproteins (RNPs); Gene Knockout Using Targeted Nucleases; Evaluation of Editing Efficiency, Targeted Integration; Donor DNA Design and Nuclease Compatibility; Detection of Integration Events, Exercises for CRISPR Design; Bioinformatics for Genome Editing Applications; Development of Genetically Modified Cell Lines Using K562 Cells (will allow attendees to run their own experiments and analyze their own results by the end of this workshop); and Best Practices for Use of the CRISPR/Cas9 System for Genome Editing.

Class size will be limited to 24

Comprehensive Binder:
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit:
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits.  For a 3-day workshop, a participant receives 2.1 CEUs.  For a 4-day workshop, a participant receives 2.8 CEUs.  For a 5-day workshop, a participant receives 3.5 CEUs. 

Refund Policy:                                
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.       
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 
 0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification:
1.  All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
2.  Cancellations received after hours are time marked for the following day.  
3.  In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
4.  All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation:
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below. 

DoubleTree by Hilton Bethesda - Washington DC

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