Skip to Main Content

IMMU 023 | Flow Cytometry : Principles and Methods

April 28, 2020 to May 1, 2020

NIH Campus- Bethesda, Maryland

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability.

NIH Fellows or NIH community members being sponsored by their lab can enroll tentatively, using the Contract: SF-182 option on the workshop’s registration site while waiting for authorization of payment. Enrollment is not guaranteed until payment is received.

Register Now

Contract: SF-182

Workshops generally run from 9:00am - 5:00pm.​

View Tentative Agenda

This four day lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for  Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Spectral Flow Cytometry; Demonstration of Miltenyi Biotec MACSQuant Analyzer, Beckman Coulter CytoFLEX, and Bio-Rad S3e™ Cell Sorter; 15 to 20 color staining (BD FACSymphony™, Beckman Coulter CytoFLEX); Bead-based Immunotechnology (Luminex, Quanterix, Cytometric Bead Array (CBA)); Hands-on Flow Cytometry Data Analysis using FlowJo.

FAES will share your email address as provided during the registration process with FlowJo, LLC who will grant you temporary FlowJo Portal access.

Lead Instructors
Pradeep K. Dagur, Ph.D. (Staff Scientist and Lab Manager, Flow Cytometry Core, NIH/NHLBI)
Kathy McKinnon (FACS Core Facility Head/Staff Scientist, NCI CCR VB FACS Core Facility)
Rafael Villasmil, M.Sc. C.Cy. (Manager & Operator, Flow cytometry core, NIH/NEI)

Field Application scientists/representative(s) of Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, and FlowJo actively provide their support in instrument demo/software hands on exercises, reagents, and teaching activities.
(*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, or FlowJo)

General Training Rate
Discounted Training Rates
$695.00 - NIH Trainees (fellows, postdocs, postbacs, working or appointed to any NIH Campus)
$895.00 - NIH Community(working or appointed to any NIH Campus)
$999.00 - Academia, US Military or US Government

Although no grades are given for BioTech workshops, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day. 
All refund payments will be processed by the start of the initial workshop.

Suggested Lodging Accommodations
A special rate is available to all workshop attendees. This includes complimentary buffet breakfast, guest room Wi-Fi, and discounted $15 parking per night. The hotel provides complimentary shuttle service to/from the NIH campus. The hotel is also within walking distance of the training facility. Reservations can be made online, see below. 

The Bethesdan by Hilton


Return to Calendar