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IMMU 023 | Flow Cytometry : Principles and Methods

January 21, 2020 to January 24, 2020

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 
NIH employees paying with the signed vendor copy of the SF-182 form, please email: 

Register Now

Contract: SF-182

Workshops generally run from 9:00am - 5:00pm.​

View Tentative Agenda

This four day lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for  Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Hands-on Laboratory with 4- and 8-color staining (Miltenyi Biotec MACSQuant Analyzer); 15 to 20 color staining (BD FACSymphony™, Beckman Coulter cytoFLEX), Data Workshop; Hands-on Data Analysis with samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers; Software training: Introduction to FlowJo and FCS Express Cytometry Analysis Software.

Lead Instructors
Pradeep K. Dagur, Ph.D. (Staff Scientist and Lab Manager, Flow Cytometry Core, NIH/NHLBI)
Kathy McKinnon (FACS Core Facility Head/Staff Scientist, NCI CCR VB FACS Core Facility)
Rafael Villasmil, M.Sc. C.Cy. (Manager & Operator, Flow cytometry core, NIH/NEI)

Field Application scientists/representative(s) of Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, Flow jo and FCS Express actively provide their support in instrument/software Demo hands-on exercises, reagents, and teaching activities.
(*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, Flow jo or FCS Express)

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% Refund or 100% Full Workshop Credit Available
- Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available
- Written Notificaiton: 4 to 13 Days prior to start of program.
 0% Refund, 100% Full Workshop Credit Available
- Written Notificaiton: 3 Days or less prior to start of program.
All cancellations must be received in writing via email to Ms. Carline Coote at 
Cancellations received after hours are time marked for the following day.
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
All refund payments will be processed by the start of the initial workshop.     

Suggested Accommodation:
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below. 

DoubleTree by Hilton Bethesda - Washington DC

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