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IMMU 023 | Flow Cytometry : Principles and Methods

October 13, 2020 to October 16, 2020

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 

NIH Fellows or NIH community members being sponsored by their lab can enroll tentatively, using the Contract: SF-182 option on the workshop’s registration site while waiting for authorization of payment. Enrollment is not guaranteed until payment is received.  

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Contract: SF-182

Workshops generally run from 9:00am - 5:00pm.​

View Tentative Agenda

This four day lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for  Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Spectral Flow Cytometry; Demonstration of Miltenyi Biotec MACSQuant Analyzer, Beckman Coulter CytoFLEX, and Bio-Rad S3e™ Cell Sorter; 15 to 20 color staining (BD FACSymphony™, Beckman Coulter CytoFLEX); Bead-based Immunotechnology (Luminex, Quanterix, Cytometric Bead Array (CBA)); Hands-on Flow Cytometry Data Analysis using FlowJo.

Lead Instructors
Pradeep K. Dagur, Ph.D. (Staff Scientist and Lab Manager, Flow Cytometry Core, NIH/NHLBI)
Kathy McKinnon (FACS Core Facility Head/Staff Scientist, NCI CCR VB FACS Core Facility)
Rafael Villasmil, M.Sc. C.Cy. (Manager & Operator, Flow cytometry core, NIH/NEI)

Field Application scientists/representative(s) of Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, and FlowJo actively provide their support in instrument demo/software hands on exercises, reagents, and teaching activities.
(*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, or FlowJo)

Although no grades are given for BioTech workshops, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Training Rate
Discounted Training Rates
$695.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$895.00 - NIH Community
$999.00 - Academia, US Government, US Military

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day. 
All refund payments will be processed by the start of the initial workshop.

Suggested Lodging Accommodations
A special rate is available to all workshop attendees. This includes complimentary buffet breakfast, guest room Wi-Fi, and discounted $15 parking per night. The hotel provides complimentary shuttle service to/from the NIH campus. The hotel is also within walking distance of the training facility. Reservations can be made online, see below.

The Bethesdan by Hilton 


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