October 13, 2020 to October 16, 2020
Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: email@example.com or call 301-496-7977 for space availability.
NIH employees paying with the signed vendor copy of the SF-182 form, please email: firstname.lastname@example.org
Workshops generally run from 9:00am - 5:00pm.
This four day lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.
Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Spectral Flow Cytometry; Hands-on Laboratory with 4- and 8-color staining (Miltenyi Biotec MACSQuant Analyzer); 15 to 20 color staining (BD FACSymphony™, Beckman Coulter CytoFLEX), Bead-based Immunotechnology (Luminex, Quanterix, Cytometric Bead Array (CBA)); Data Workshop; Hands-on Data Analysis with samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers; Software training: Introduction to FlowJo and FCS Express Cytometry Analysis Software.
Pradeep K. Dagur, Ph.D. (Staff Scientist and Lab Manager, Flow Cytometry Core, NIH/NHLBI)
Kathy McKinnon (FACS Core Facility Head/Staff Scientist, NCI CCR VB FACS Core Facility)
Rafael Villasmil, M.Sc. C.Cy. (Manager & Operator, Flow cytometry core, NIH/NEI)
Field Application scientists/representative(s) of Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, FlowJo and De Novo Software actively provide their support in instrument/software Demo hands-on exercises, reagents, and teaching activities.
(*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from Beckman Coulter, Bio-Rad, Luminex, Miltenyi, BioLegend, FlowJo or De Novo Software)
Contains all notes, protocols, and reference material needed. No additional books are required.
Although no grades are given for BioTech workshops, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.
100% tuition refund for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 days or less prior to the start of the workshop.
All cancellations must be received in writing via email to Ms. Carline Coote at email@example.com.
Cancellations received after hours are time marked for the following day.
All refund payments will be processed by the start of the initial workshop.
Suggested Lodging Accommodations
A special rate is available to all workshop attendees. This includes complimentary buffet breakfast, guest room Wi-Fi, and discounted $15 parking per night. The hotel provides complimentary shuttle service to/from the NIH campus. The hotel is also within walking distance of the training facility. Reservations can be made online, see below.