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BioTech 100 | Single Cell RNA Library Preparation for Next Generation Sequencing (NGS) | 2-Day Hands On Workshop

March 19, 2019 to March 20, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 

NIH employees paying with the signed vendor copy of the SF-182 form, please email:

Register Now

Contract: SF-182

This is a two day hands-on lab training event to prepare Single Cell and Low Input RNA-Seq libraries for Illumina Next Generation Sequencing under the instruction of experts from the industry.
Specifically, participants will prepare single cell RNA and low input RNA sequencing libraries using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®.

The training will include:
•    Hands-on single cell and low input RNA library preparation
•    cDNA and Library QC by Agilent Tape Station
•    Discussions regarding the Bulk vs Single cell RNA-seq. Single cell RNA library prep workflow, chemistry, expected sequencing data performance and troubleshooting strategies.

What to bring?
Isolated HeLa cells and Low input RNA samples will be provided.

Participants should be comfortable with basic computer skills.

Class size is limited to 20

Other FAES@NIH workshops relevant to RNAseq/NGS

BioTech 45 | Bioinformatics Analysis of Next Generation Sequencing Data

BioTech 74 | Advanced Transcriptomics (RNA Seq) Analysis

BioTech 75 | Metagenomics Data Analysis

(*This workshop is purely educational/training. FAES@NIH does not endorse any specific company/product; nor do not have any financial agreements/commission from NEB)

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours.   One CEU is equal to ten contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Refund Policy
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.  
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop.
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop. 

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after hours are time marked for the following day.
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation:
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below.

DoubleTree by Hilton Bethesda - Washington DC

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