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BioTech 29 | Laser Capture Microdissection: Methods for Microgenomic Analysis | 5-Day Hands On Workshop

October 15, 2018 to October 19, 2018

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: training@faes.org or call 301-496-7977 for space availability. 

NIH Only: Payment approval and authorization is done through your AO or lab manager. Receiving lab approval does not constitute enrollment. Fellows or those being sponsored by their lab can enroll using this form while waiting for authorization of payment.

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Contract: SF-182

Laser Microdissection systems allow for the procurement of specific populations of cells from tissue and cytology and live cell culture samples containing heterogeneous populations of cells. The specificity of analyses is therefore much more representative of the disease process being studied. This approach to microdissection ensures that biological molecules, such as RNA and DNA and proteins, remain undamaged during the microdissection process. Downstream molecular analysis of these molecules produces accurate and assured results that have led to over 2,000 peer-reviewed publications by independent researchers.

In this five-day training program, participants learn to prepare tissue specimens for microdissection, then select and acquire homogenous cell populations using the mmi-CellCut, Leica LMD, Arcturus XT, and PALM microdissection systems. Instruction emphasizes operation of these LM systems, appropriate tissue handling and sample preparation for subsequent DNA, RNA or protein analysis, and methods for proper molecular extraction. Lecture and detailed instructions to prepare samples for several downstream molecular analyses are presented.

Workshops generally run from 9:00am - 5:00pm.​

View Tentative Agenda

Lecture and Laboratory Topics
Overview of Laser Microdissection Technology (History, Theory, Applications); Tissue Sample and Slide preparation; Project set up and QC of microdissected samples, genomic analysis, mRNA analysis including quantitative RT-PCR gene expression analysis and microarrays from microdissected tissue samples, microRNA analysis from microdissected samples, and proteomic analysis from frozen and formalin-fixed, paraffin-embedded microdissected samples using Mass Spec. Special microdissection techniques such as immuno-guided LM and live cells dissections will be also covered.  The lectures also include examples of actual scientific and clinical applications of LM-based studies. The hands on sessions include tissue slide preparation and histology review for LM, and practice on Arcturus XT, PALM, Leica and mmi-CellCut platforms.

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional 
books are required. 

Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours.   One CEU is equal to ten contact hours.   Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Refund Policy                                                                                                             
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 
0% refund, 100% full workshop credit is available for registrations cancelled 
3 days or less prior to the start of the workshop.  

Notification
All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov
Cancellations received after hours are time marked for the following day.  
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation:
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below. 

DoubleTree by Hilton Bethesda - Washington DC

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