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BioTech 3 | Methods in Molecular Biology

May 20, 2019 to May 23, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 

NIH Only: Payment approval and authorization is done through your AO or lab manager. Receiving lab approval does not constitute enrollment. Fellows or those being sponsored by their lab can enroll using this form while waiting for authorization of payment.

Register Now

Contract: SF-182

This 4 day lecture and hands-on laboratory course is designed to provide the novice with an introduction to the molecular biology and related laboratory procedures which are widely used in biotechnology. The participants in this workshop  will acquire skill in the use of "generic" methods, and through an understanding of such methods, improve their effectiveness as workers in a contemporary research laboratory.

Lecture Topics
Basics of mammalian & bacterial cell culture and cell maintenance. Types of cell lines, differences between cell line and primary culture; Introduction Nucleic Acids; DNA and RNA extraction and Purification. Methods to quantify and analyze DNA and RNA.  Protein Purification & Analysis; Basics of genetic engineering: Restriction Enzymes and Restriction Mapping; Other Pertinent Enzymes; Cloning end expression vectors, DNA libraries, libraries screening libraries; The Polymerase Chain Reaction Part I: Principles, Theory, Optimization; The Polymerase Chain Reaction Part II – PCR Variations: Real Time PCR, qPCR, Nested PCR

Laboratory Topics
Lab basics: Pipetting; Cell culture techniques; primer design, mapping and molecular cloning; restriction enzyme digestion of recombinant plasmid and ligation; Nucleic Acids isolation, purification, and quantification; bacterial transformation, reverse transcription PCR, quantitative real time PCR; SDS-PAGE and Western blot; Bioinformatics tools

General Training Rate*
*10% Group discount available when 3 or more participants register together from the same organization.

Discounted Training Rates
$695.00 - NIH Trainees (Fellows, Postdocs, Postbacs, Grad Students working at the NIH Campus) 
$895.00 - NIH Community (Working, Appointed, or Assigned to the NIH Campus)
$995.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% Refund or 100% Full Workshop Credit Available
Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available
Written Notificaiton: 4 to 13 days prior to start of program.
 0% Refund, 100% Full Workshop Credit Available
Written Notificaiton: 3 days or less prior to start of program.
All cancellations must be received in writing via email to Ms. Carline Coote at 
Cancellations received after hours are time marked for the following day.
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
All refund payments will be processed by the start of the initial workshop.

DoubleTree by Hilton Bethesda - Washington DC

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