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CellServ

FAES offers innovative, inexpensive self contained kits and PowerPoint presentations.

FAES offers innovative, inexpensive self contained kits and PowerPoint presentations for hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

Click here to order CellServ Kits and PowerPoint Presentations to enhance your class. 

CellServ responds to the pressing need of forward-looking instructors who want to incorporate exciting, novel, cell-culture based exercises into their laboratory programs. Each topic addressed by a CellServ Kit, which serves 30 students, provides instructors with an opportunity to expand on the importance of the biological principles associated with the topic at hand. CellServ offers cost-effective materials and instructions that:

  • familiarize students with the underlying principles of cell culture technology and its application to diverse areas of research,
  • permit students to participate in a uniquely different and important technology,
  • allow students to sharpen their observation and quantitative microscopy skills and
  • reduce instructor preparation time.

CellServ also offers an extensive line of PowerPoint Presentations on varied core biotechnology topics to enhance the learning experience of students without increasing the burden on teachers.

Payment Options

Payment can be made below online (We do not accept AMEX).
If you wish to pay by check or by PO please email PO request to cellserv@faes.org for processing.
We can assist you at this email with all your questions.

CellServ Kits

Please Note:
All CellServ products are shipped out on Mondays. When ordering Kits 4 and 5, please understand that these kits are perishable and time sensitive! Due to the perishable nature of the cells, Kit #4 and Kit #5 MUST be shipped by FedEx 2nd Day or FedEx Overnight and are good for only 2-4 weeks when stored at  0 to -20 Celsius

PowerPoint Presentations

Please Note:
Each 35-45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35-40 minute class period.

CellServ Kit Descriptions

CSK1 - Visualization of Normal and Tumor Cells

Kit 1: Visualization of Normal & Transformed Cells

Using this kit, each student will be led through a step by step procedure which involves staining, mounting, and observation of normal and transformed (tumor) cells. Each student will observe the morphological differences, as well as the differing growth patterns of these cell types when grown in culture.

The manual provided with this kit describes how the cells are grown in culture, what factors influence the transformation of cells, and the differences in growth behavior between normal and tumor cells in culture. Photographs of these cell types are used to guide the students through the observation steps

L929 transformed mouse cells
Suitable for introductory biology, cytology, cell culture, oncology, and developmental biology courses.

$75.00 Each Kit includes

  • Enough materials for 30 students working individually.
  • Coverslips with IMR-90, a normal, diploid human cell line which shows the typical fibroblastic morphology and oriented growth pattern
  • Coverslips with L929, a transformed mouse cell which shows cells of varying morphology and giant cells.
  • STAIN #1 and STAIN #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Monocular or binocular microscope (10 - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

30-45 minutes, including casual observations.

These are innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK2 - Observation of Heterokaryons Through Cell Fusion

Kit 2: Observation of Heterokaryons Through Cell Fusion

Cell fusion has played an important role in gene mapping and hybridoma formation. This kit contains cells of two different species (mouse L929 cells and human fibroblasts) which have been fused and fixed to coverslips prior to shipping. Following a staining and mounting procedure, each student will observe the fused cells and tabulate the incidence of successful heterokaryon formation (mouse cell with human cell).

The manual discusses the methods by which cells are fused and the practical applications of this technology by biomedical researchers. This kit provides a unique way of introducing somatic cell genetics and hybridoma technology and their applications.

Cell fusion has played an important role in gene mapping and hybridoma formation. This kit contains cells of two different species (mouse L929 cells and human fibroblasts) which have been fused and fixed to coverslips prior to shipping. Following a staining and mounting procedure, each student will observe the fused cells and tabulate the incidence of successful heterokaryon formation (mouse cell with human cell).

The manual discusses the methods by which cells are fused and the practical applications of this technology by biomedical researchers. This kit provides a unique way of introducing somatic cell genetics and hybridoma technology and their applications.

IMR-90 mormal diploid human fibroblast cell line
Suitable for introductory biology, genetics, and immunology courses.

$75.00 Each Kit includes

  • Enough materials for 30 students working individually
  • IMR-90 cells, a normal diploid human fibroblast cell line fixed to coverslips
  • L929, a transformed mouse cell line, fixed to coverslips.
  • STAIN #1 and STAIN #2.
  • Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, and a Further Reading Section.

Teacher's Preparation

  1. Monocular or binocular microscope (10 - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

60-80 minutes, including casual observations and calculations. There is a logical stopping point for those who are not on block schedule.

Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK3 - Effects of Cytotoxins on Cells

Kit 3: Effects of Toxic Chemicals on Cells

With this kit, each student will assess the various types and levels of abnormalities which may arise in cells following exposure to a cytotoxin. Mouse L929 cells are grown on coverslips in the presence of both low and high levels of colchicine. Each student will stain and mount the cells and observe with a microscope the effects of the toxin on cell morphology. Students compare these aberrant morphologies with those of untreated cells.

This exercise provides a practical introduction to the field of in vitro toxicology and its importance in biomedical research and environmental science.

The manual provides insights into the practical applications of this important field of study. The accompanying photographs illustrate some typical examples of cellular aberrations.

Suitable for introductory biology, cell biology, cytopathology, and environmental science courses.

$75.00 Each Kit includes

  • Enough materials for 30 students working individually
  • L929 cells, a transformed mouse cell line, fixed to coverslips: zero dose.
  • L929 cells exposed to 1µg/ml of colchicine and fixed to coverslips
  • L929 cells exposed to 10µg/ml of colchicine and fixed to coverslips
  • STAIN #1 and STAIN #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Monocular or binocular microscope (10X - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

60-80 minutes, including casual observations and calculations.

CSK4 - Preparation of Human Chromosome Spreads HeLa Cells

Kit 4: Preparation of Human Chromosome Spreads HeLa Cells

Using this kit, each student will be provided the unique opportunity of preparing a chromosome spread using human cells. The excitement generated by the students as they "splat" the cells onto microscope slides and proceed to "hunt" for the ideal chromosome spread with the microscope has generated an equal level of excitement among the hundreds of teachers who use this kit. Because the cells used for this exercise are transformed, the number of chromosomes will vary among the cells. Each student can count the chromosomes and see firsthand the range of aneuploidy. These are HeLa cells

The manual provides information regarding chromosome structure and the important role of karyotyping in geneticanalysis and genetic disease diagnosis.

Suitable for introductory biology, genetics, cytogenetics, cell biology, and oncology courses.

$75.00 Each Kit includes

  • Enough materials for 30 students working individually.
  • 15 tubes each containing metaphase-blocked cell suspension, fixed in acetic acid-methanol fixative.
  • Stain #1 and Stain #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion) will enable the students to identify and count chromosomes ans see sister chromatids.
  2. Pipettes, microscope slides, coverslips.
  3. Staining jars or beakers ( = 250ml) containing room temperature water.

Time Requirements

40-60 minutes.

Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK5 - Mouse Chromosome Studies

Kit 5: Mouse Chromosome Studies

Familiarity with mouse chromosomes is warranted because of the pivotal role of the mouse as an experimental organism and its importance in comparative karyotyping which serves to assist in the identification and mapping of human genes.

Kit 5, Mouse Chromosome Studies, is designed to introduce students to the basic principles entailed in the manipulation of mammalian cells so that chromosomes may be readily visualized, identified and studied. As mouse cells are cultured, their karyotype becomes varied due to an accumulation of translocations and other structural aberrations. Hence the cells in this kit provide a unique opportunity for the students to identify different structural aberrations and try to explain their origins.

Fixed swollen cells arrested in metaphase are provided for student use. The student will affix the cells to microscope slides and stain. These slides are scanned for good chromosome spreads which are observed at high magnification. Students search for normal and aberrant chromosomes, identifying the position of the centromere and relative sizes of chromosome arms.

The manual provided will describe how the cells were prepared for karyotyping, and subsequent steps exercised by the students. It also describes chromosome structure and structural aberrations.

Suitable for introductory biology, genetics, cytogenetics, cell biology, and oncology courses.

$75.00 Each Kit includes

  • Enough materials for 30 students working individually.
  • 15 tubes each containing metaphase-blocked mouse cell suspension, fixed in acetic acid-methanol fixative.
  • Stain #1 and Stain #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion) will enable the students to identify and count chromosomes ans see sister chromatids.
  2. Pipettes, microscope slides, coverslips.
  3. Staining jars or beakers ( = 250ml) containing room temperature water.

Time Requirements

40-60 minutes.

 

PowerPoint Presentation Descriptions

CSPP1 Normal and Cancer Cells In Vitro

PowerPoint Presentation Topics

  • The Importance of in vitro Technology: Cell, Tissue and Organ Culture Defined
  • Use for Basic and Medical Research: Gene Mapping, Genetic Disease, Growth Factors, The Cell Cycle, Carcinogenesis
  • From Biopsy to Culture: Importance of Sterility, Biopsy vs. Whole Animal Embryo as a Cell Source, Tissue Mincing, Tissue Dissociation with Proteolytic Enzymes, The Primary Culture - Survival, Proliferation, and Elimination
  • The In Vitro Environment: Physical Environment & Chemical Environment
  • Normal Cells: Formation, Attachment, Contact Inhibition, Finite Life Span, and Senescence
  • Cancer Cells: In Vivo Behavior, In Vitro Properties, Evaluation of Tumorigenic Potential, Immortal Cells, Chromosome Abnormalities.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP2 Polymerase Chain Reaction (PCR)

PowerPoint Presentation Topics

  • The Polymerase Chain Reaction (PCR) Defined
  • The Birth of PCR
  • Molecular Events of the Cycle: Denaturation, Primer Annealing, Primer Extension
  • Final Extension
  • Oligonucleotide Selection: Primer Design and Usage
  • Primer Length and Melting Temperature (Tm)
  • Base Composition
  • Using BLAST for Assessing Primer Design
  • Using the Internet and Software Programs for Primer Design and Usage
  • Properties of Thermostable DNA Polymerases
  • Optimization of the Reaction: A Typical Reaction Mix, Importance of Mg2+ Concentration, Guidelines for Determining Number of Cycles, PCR Additives for Optimization
  • PCR Contamination: Prevention and Control: Variations on the PCR Theme.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP3 Principles of Gel Electrophoresis

PowerPoint Presentation Topics

  • General Considerations and Principles: The Electrophoretic Matrix: Properties of Agarose and Acrylamide
  • An Introduction to the theory of Electrophoresis: Interrelationship of Resistance, Voltage, Current and Power, Electrolysis and pH Effects
  • Nucleic Acid vs. Protein Separation
  • Denaturing vs. Non-Denaturing (Native) Electrophoresis
  • Sample Preparation; Comparison of Buffer Systems for Nucleic Acid Separations
  • Comparison of Buffer Systems for Protein Separation
  • Detection of Nucleic Acids in Agarose and Acrylamide Gels
  • Detection of Nucleic Acids on Blot Transfer Membranes
  • Detection of Proteins in Acrylamide Gels
  • Detection of Proteins on Blot Transfer Membranes.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP4  KaryotypingCSPP5 Cell Fusion

PowerPoint Presentation Topics

  • Karyotyping Defined
  • Representative Applications in Biomedical Studies
  • Chromosome Structure and Changes During the Cell Cycle
  • The Human Karyotype: Homologous Chromosomes, Somatic & Sex Chromosomes, and Chromosome Nomenclature
  • Manipulation of Cultured Cells for Karyotyping
  • Banded Chromosomes
  • Human Genome Project and Mapping of Disease and Normal Genes

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP5 Cell Fusion

A Powerful Tool for Monoclonal Antibody Production, Somatic Cell Genetics and Gene Mapping to Chromosomes

PowerPoint Presentation Topics

  • Cell Fusion-- from Cytopathology Observation to Controlled Experimentation
  • The Age of Manipulative Biology and the Deliberate Manufacture of Somatic Cell Intra-species and Inter-species Hybrids
  • Overview: Fusion Partners (Parental Cells), The Fusion Process from Heterokaryons to Hybrids, The HAT Selection System
  • Complementary Parental Cells for TK and HGPRT genes
  • Hybrid Cell Line Stability and Random Loss of Genes
  • Alternative Fusion Methods
  • Use of Hybrids in Cancer Research: Fusion of Normal and Cancer Cells
  • Fusion of Antibody Producing Cells and Tumor Cells to Obtain an Immortal Hybridoma Cell Line Secreting One Antibody (Monoclonal Antibody Production)
  • Hybrid Cell Lines and Gene Mapping: Fusion Partners, Clonal Lines with Varying Karyotype, Correlation of Loss of Biochemical Trait with Loss of Specific Chromosome
  • Cell Reconstruction: The Assembly of Functional Cells with Organelles from Different Cells

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP6 Toxicology and Toxicity Testing

PowerPoint Presentation Topics

  • Toxicology as a Science: Basic Research Goals and Application to Environmental Safety
  • The Imperative and Mandate for Chemical Testing
  • Toxicology Strategies: Dose and End-point for Toxicity
  • Whole Animal Models: Draize Test, LC50 Assay, Carcinogenesis, Developmental Toxicity
  • In Vitro Toxicology as a Complement to Animal Studies: Is it an Alternative?
  • Common in vitro Tests: Genotoxicity (Mutangenesis), Cell Transformation/ Carcinogenesis, Chorioallantoic Membrane Assay, Cytotoxicity Assays
  • Biotransformation- Metabolic Activation: Need for Supplementation
  • In Vitro-In Vivo Test Systems
  • Use of In Vitro Test Data in Decision Making

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CellServ has been in existence since 1989 and has served over 450,000 student-lab units nationwide. It has been adopted by several school districts and is recommended in several published manuals. Attesting to the value of the instructional material is the repeated and expanding use of the service.