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BIOC 035 | Imaging – From IF and FISH to Automated and Confocal Microscopy

August 10, 2020 to August 14, 2020


Registration occurs on a firstcome, firstserved basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 

NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. FAES must receive payment within 7 business days after reserving a seat or 3 business days before the start of the workshop, which ever comes first. If payment is not received in this time frame, your reservation will be canceled.

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Reserve a Seat

This five-day online workshop will utilize a combination of lectures and remote Q&A lab sessions in a “boot camp”-like approach for cell-biologists who seek to learn imaging techniques used extensively in biomedical research. This course will first begin with training in immunofluorescence, in situ hybridization (RNAscope), and cell transfection with an early introduction to automated imaging and widefield fluorescence microscopy. The course will then fully focus on the world of confocal microscopy, a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components. Through interactive online lectures and labs participants will be introduced to the most commonly used confocal microscopy techniques in addition to extensive training in image analysis using Fiji. Participants will also have interactive remote sessions with highly qualified scientists from Leica, Nikon, Olympus and Zeiss, where they will receive training in microscope basics and imaging techniques on each individual platform.

Lecture Topics

Introduction to Fluorescence Microscopy
Immunofluorescence (with workflows)
RNAScope (with workflows)
Cell Transfection for Fixed and Live Cell Imaging (with workflows)
High throughput Screening and Automated Imaging
Confocal Microscopy
Light sheet Microscopy
2-Photon Excitation
Introduction to Super-resolution Imaging (Airyscan, SIM, STED, SMLM)
Image Processing and Analysis using Fiji
Colocalization of Proteins
Spectral Unmixing

Remote Lab Topics
Colocalization using multi-color fluorescence imaging
Spectral Unmixing
Protein Dynamics Using FRAP, FLIP, and PA-GFP
Image Processing and Analysis using Fiji

Who Should Attend?
Biomedical Scientists just entering the world of imaging and confocal microscopy. 

Faculty Members & Instructors
Instructors are leading experts in the field from both academia and industry.  

Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits.  For a 3-day workshop, a participant receives 2.1 CEUs.  For a 4-day workshop, a participant receives 2.8 CEUs.  For a 5-day workshop, a participant receives 3.5 CEUs. 

Training Rate
Discounted Training Rates
$795.00 - NIH Trainees (Fellows, PostDocs, PostBacs working at the NIH Campus)
$995.00 - NIH Community (Working, Appointed, or Assigned to the NIH Campus)
$1,099.00 - Academia, US Government, US Military 

Refund Policy
100% tuition refund for registrations canceled 14 or more days prior to the start of the workshop.
50% tuition refund for registrations canceled between 4 to 13 days prior to the start of the workshop.
No refund will be issued for registrations canceled 3 days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after 4:00 PM on business days are time marked for the following day. 
All refund payments will be processed by the start of the initial workshop. 

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