April 13, 2020 to April 17, 2020
NIH Campus- Bethesda, Maryland
Registration occurs on a firstcome, firstserved basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: firstname.lastname@example.org or call 301-496-7977 for space availability.
NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. Payments need to be received within 7 business days from date of reservation or before the start of a workshop, whichever comes first. Seat reservations will be cancelled if payment is not received prior to workshop.
This five-day workshop will utilize a combination of lectures and hands-on lab sessions in a “boot camp”-like approach for cell-biologists who seek to learn imaging techniques used extensively in biomedical research. This course will first begin with training in immunofluorescence, in situ hybridization (RNAscope), and cell transfection with an early introduction to automated imaging and widefield fluorescence microscopy. The course will then fully focus on the world of confocal microscopy, a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components. Participants will study both fixed and live specimens, on commercially available equipment.
Introduction to Fluorescence Microscopy
In Situ Hybridization
Cell Transfection for Fixed and Live Cell Imaging, High throughput Screening and Automated Imaging
2-Photon Excitation; Colocalization of Proteins
FRAP and FLIP
Image Processing and Analysis using Fiji
SMLM (GSD, PALM, STORM, PAINT)
Fixed Sample Preparation for Fluorescence/Confocal Microscopy
Cell Transfection for Fixed and Live Cell Imaging
Image Acquisition and Analysis with Automated Widefield Microscopes
Acquisition/Optimization of Basic Confocal Scans using Single-point Laser-Scanning Confocal Microscopes
Multiphoton excitation, and Spinning Disk Confocal
Colocalization using multi-color fluorescence imaging
FRET; Spectral Unmixing
Live Cell Imaging
Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging
Protein Dynamics Using FRAP, FLIP, and PA-GFP
Image Processing and Analysis using Fiji
Who Should Attend?
Biomedical Scientists just entering the world of imaging and confocal microscopy.
Faculty Members & Instructors
Instructors are leading experts in the field from both academia and industry.
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits. For a 3-day workshop, a participant receives 2.1 CEUs. For a 4-day workshop, a participant receives 2.8 CEUs. For a 5-day workshop, a participant receives 3.5 CEUs.
Discounted Training Rates
$795.00 - NIH Trainees (Fellows, PostDocs, PostBacs working at the NIH Campus)
$995.00 - NIH Community (Working, Appointed, or Assigned to the NIH Campus)
$1,099.00 - Academia, US Government, US Military
100% tuition refund for registrations canceled 14 or more days prior to the start of the workshop.
50% tuition refund for registrations canceled between 4 to 13 days prior to the start of the workshop.
No refund will be issued for registrations canceled 3 days or less prior to the start of the workshop.
All cancellations must be received in writing via email to Ms. Carline Coote at email@example.com.
Cancellations received after 4:00 PM on business days are time marked for the following day.
All refund payments will be processed by the start of the initial workshop.
Suggested Lodging Accommodations
The Bethesdan by Hilton Hotel (formerly DoubleTree Bethesda)
8120 Wisconsin Avenue
Bethesda, MD 20814
A special seasonal rate plus 13% tax for all workshop attendees is available at The Bethesdan by Hilton Hotel. The rate includes complimentary in-room WiFi, breakfast buffet, $15 discounted overnight parking, and complimentary shuttle to and from the NIH campus.
A credit card will be required to book the reservation. Cancellations must be made 48 hours prior to scheduled arrival to avoid a no-show charge. Check-in time is 3:00 p.m.; check-out is 12:00 p.m.
Book Online: The Bethesdan by Hilton
- Location and Transportation
The Bethesdan is located within walking distance (0.8 miles) from the NIH main campus. Please inquire with the hotel directly regarding their shuttle schedule to/from the campus and other hotel amenities. The nearest Metro subway stops to the hotel are the Bethesda station (1 mile South of The Bethesdan) and Medical Center station (1 mile North of The Bethesdan), both on the Red line.
Access to the NIH Campus
All NIH visitors must enter through the Visitor’s Gateway Center (Bldg. 66) located at 9000 Rockville Pike-Rt. 355/Wisconsin Ave., Bethesda, MD. Taxi, bus, personal auto, and Uber/Lyft drop-off as well as the Medical Center (Red line) Metro subway station, a visitor’s parking garage (MLP-11), and access to the NIH campus shuttles are all located at this facility. Parking at the MLP-11 visitor’s garage is $12/day.
NIH Security Screening
Please arrive early at the Visitor’s Gateway to allow for security screening which could take 10 to 30 minutes depending on the volume of campus visitors. You MUST show a valid government-issued photo ID (driver's license or passport). Please visit the NIH website for helpful Visitor Information as well as directions, maps, campus shuttle schedules, and security screening information.