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BIOF 045 | Bioinformatic Analysis of Next Generation Sequencing Data

July 28, 2020 to July 31, 2020


Registration is closed.

Registration occurs on a first-come, first-served basis. Programs are open to general public. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7975 for space availability. 

NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. FAES must receive payment within 7 business days after reserving a seat or 3 business days before the start of the workshop, which ever comes first. If payment is not received in this time frame, your reservation will be canceled.

Next generation sequencing technologies are producing enormous amount of sequencing data. Analyzing this massive amount of data requires the ability to use the sophisticated tools and techniques in the Unix platform. This course will introduce the participants to the skills needed to get started with their NGS data analysis journey.

Simultaneous access to two screens is highly recommended for adequate learning experience. Examples include two laptops, one computer with two screens, one laptop and one tablet, etc.

Participants will start with a general introduction to the NGS data analysis concepts, methods and tools, followed by a gentle biologist friendly Linux hands-on training.
An NGS expert will then walk through
a. RNA-Seq analysis starting with QC, preprocessing, alignment, transcript quantification, differential expression and downstream analysis/visualization 
b. DNA-Seq variant calling analysis with QC, alignment, variant calling, variant filtering and annotation
c. ChIP-Seq analysis with peak calling, peak annotation, motif analysis
d. Integration of NGS data using Network analysis methods and tools.


  • Participants will use a Graphic User Interface based Linux Desktop environment, specially configured for NGS data analysis in the cloud
  • Training provided by experienced active NIH researchers
  • Cookbook style bound manual for all exercises
  • Continuing Education Credits

Hands-on Skills/Tools taught
Basic Linux Skills
QC: FastQC
Pre-Processing: Trimmomatic
Alignment: BWA, HISAT2
Transcript Quantification: StringTie
Differential Expression Analysis: BallGown
Visualization: Samtools, RStudio
Variant Calling: GATK
Variant Annotation: VEP
Peak Calling: MACS2
Motif Finding: MEME
Downstream Analysis: UCSC Table Browser, DAVID
Network Analysis: Cytoscape, STRING
Utilities: Picard, Samtools, Galaxy, BCFTools

Who should attend

  • Researchers and academics who are interested in analysing and exploring the NGS data, like RNA-Seq, DNA-Seq and ChIP-Seq data.
  • Clinicians who are interested in discovering novel biological regulatory mechanisms looking for potential diagnostic biomarkers and therapeutic uses.
  • Biomedical students who are interested in pursuing research on transcriptomics, genotyping, epigenomics etc.
  • Scientists in program administration interested in learning and understanding the latest methods and concepts in NGS data analysis.

Participants should be comfortable with basic computer skills.

    General Training Rate
    Discounted Training Rates                                                                           
    $695.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
    $895.00 - NIH Community                                                                               
    $999.00 - Academia, US Government, US Military  

    Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours.   One CEU is equal to ten contact hours.   Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

    Refund Policy
    100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
    50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
    No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

    All cancellations must be received in writing via email to Ms. Carline Coote at
    Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day. 
    All refund payments will be processed by the start of the initial workshop.

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