January 23, 2018 to January 26, 2018
Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: firstname.lastname@example.org or call 301-496-7977 for space availability.
NIH Only: Payment approval and authorization is done through your AO or lab manager. Receiving lab approval does not constitute enrollment. Fellows or those being sponsored by their lab can enroll using this form while waiting for authorization of payment.
Workshops generally run from 9:00am - 5:00pm.
Through morning classes and afternoon "hands-on" lab sessions, Biotech 35 will focus on two important methods that are used extensively in biomedical research. Fluorescence microscopy is a useful tool for observing cellular morphology and function that is readily available and relatively simple to learn. Confocal microscopy has emerged as a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components. The course is designed as a “boot camp” for those cell biologists just entering the world of confocal microscopy, wishing to utilize the technology to its fullest potential. The course is divided roughly as 30% class, 15% wet lab, 30% microscopy, and 15% image processing/analysis. Advanced topics are introduced to give participants an inkling of future possibilities.
Basic Theory and Practical Aspects of Fluorescence Microscopy, Immunofluorescence, and Confocal Microscopy; Specimen Preparation; Spinning Disk Confocal; Super Resolution Confocal; 3D Deconvolution; Colocalization of Proteins: Using FRET to show protein-protein interactions; Spectral Unmixing; Live Cell Studies: Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging; Protein Dynamics Using FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching), and PA-GFP (Photo-Activatible Green Fluorescent Protein); Image Processing and Analysis using Fiji. SIM (Structured Ilumination Microscopy) will be introduced without an accompanying lab.
Participants will gain hands-on experience with many of the topics introduced in class: Sample preparation for Fluorescence/Confocal Microscopy; Acquisition/Optimization of Basic Confocal Scans using Single-point Laser-Scanning Confocal Microscopes, and Spinning Disk Confocal; Colocalization using multi-color fluorescence imaging; FRET; Spectral Unmixing; Live Cell Imaging: Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging; Protein Dynamics Using FRAP and/or FLIP; Image Processing and Analysis using Fiji. Every participant will be performing the wet labs and the computer labs individually, concurrently with others; whereas microscopy labs, conducted in groups of 4 or less, will share time among the participants.
Related Bio-Tech Course
BioTech 62: Image Processing and Analysis
BioTech 53: Super Resolution Microscopy
10% group discount available when 3 or more participants register together from the same organization
$595.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$895.00 - NIH Community
$995.00 - Academia, Government, Military
Comprehensive Binder Included
Contains all notes, protocols, and reference material needed. No additional books are required.
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop.
0% refund, 100% full workshop credit is available for registrations cancelled
3 days or less prior to the start of the workshop.