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BioTech 53 | Super Resolution Microscopy | 4-Day Hands-On Workshop

March 20, 2018 to March 23, 2018

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability.
NIH employees paying with the signed vendor copy of the SF-182 form, please email:

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Workshops generally run from 9:00am - 5:00pm.​​

View Tentative Agenda

Super Resolution Microscopy represents a group of recently developed light microscopic techniques that are able to exceed diffraction-limited resolution (less than 200nm). This course will focus on three types of Super Resolution Microscopy- Structured Illumination Microscopy (SIM), Stochastic Optical Reconstruction Microscopy (STORM), and Stimulated Emission Depletion (STED).  In addition, students will be exposed to cutting-edge super resolution microscopes developed at HHMI Janelia Research Center through the AIC (Advanced Imaging Center).  The AIC will showcase several instruments, including iPALM, lattice light sheet and live-cell TIRF-SIM.  

The course is designed for cell biologists with prior experience in light microscopy who wish to add super resolution microscopy to their research program. Participants will acquire both theoretical understanding of super resolution microscopy and practical experience using state-of-the-art super resolution microscopes.

Lecture Topics
Introduction to Super Resolution Microscopy; Advances in Super Resolution Microscopy; Theoretical Background of SIM, PALM/dSTORM, and STED Imaging; Advances in Fluorescent Protein and Organic Dye Technology; Applications of Super Resolution Microscopy using SIM and dSTORM; Challenges Associated with Obtaining Good SIM, STORM and STED Images; Potential Artifacts Common to Super Resolution Imaging.

Laboratory Topics
Working in groups of five, participants will rotate through multiple work stations. 
The following work stations are located on NIH campus: 
- Leica gSTED, GE- OMX SIM, Nikon N-STORM, Zeiss Elyra PALM/SIM, Zeiss LSM880-AiryScan, Shroff Lab - Instant SIM, Shroff Lab - Dual View Plane Illumination Microscope.  

Another three stations are located on Janelia Farm campus including iPALM, Lattice light sheet microscope, TIRF-SIM for live cell imaging.  Complimentary transportation will be provided to and from the Janelia Farm campus.  

During the laboratory sessions, the following topics will be covered:
-   Introduction and Feature Highlights of the Instrument
-   Imaging Acquisition Procedures 
-   Sample Preparation Requirements and Recommendations (Dye Choices and Imaging Solutions)
-   Imaging Data Analysis and Presentation
-   Trouble-shoot Common Problems in SIM Imaging
-   Acquisition of 2D and 3D STORM Images

Related BioTech Course
BioTech 35: Confocal & Immunofluorescence Microscopy

General Training Rate

Discounted Training Rates
$645.00 - NIH Trainees (Fellows, Postdocs, Postbacs working at  any NIH Campus)
$945.00 - NIH Community (Working, Assigned or Appointed to any NIH Campus)
$1045.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs.

Refund Policy

100% Refund or 100% Full Workshop Credit Available                                 
Written Notification: 14 or more days prior to start of program. 

50% Refund or 100% Full Workshop Credit Available
Written Notification: 4 to 13 days prior to start of program.                                           

0% Refund, 100% Full Workshop Credit Available                                             
3 days or less prior to start of program.

All cancellations must be received in writing via email to Ms. Carline Coote at

Cancellations received after hours are time marked for the following day.

In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.

All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below. 

DoubleTree by Hilton Bethesda - Washington DC


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