July 23, 2018 to July 27, 2018
Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: firstname.lastname@example.org or call 301-496-7977 for space availability.
NIH employees paying with the signed vendor copy of the SF-182 form, please email: email@example.com
Workshops generally run from 9:00am - 5:00pm.
Gene engineering provides the ability to manipulate gene expression in a desired cell type. In order to realize the full potential of stem cells, the development of tools to modify targeted genes is paramount. This course will provide an overview of three different engineering platforms including CRISPR, TALENs, and ZFNs.
The first part of the course will cover the general principles of each of these technologies including design and assembly along with the platforms available and different costs associated with each of them. The second part of the course will transition into different applications including engineering in mice, disease modeling, generating iPSC reporter lines, and high throughput approaches.
We will also consider sequencing and quality control considerations for these technologies. Hands on laboratory exercises will accompany lectures to provide training in design, assembly, transfection, and confirmation assays.
General Principles of Engineering and Disease Models; TALEN-mediated Gene Correction in iPSCs; DNA Repair Mechanisms; Targeted Mutagenesis in Zebrafish using ZFNs and TALENs; CRISPR Engineering in EC Cells and Oocytes for Mouse Model; Targeting Multiple Genes Using CRISPR in Zebrafish; Engineering Mice Using CRISPR/Cas9; Developing Reporter Lines; Panel Discussion to Discuss Current Research Projects; Safe Harbor Gene Targeting of iPSC or Hematopoietic Stem Cells to Correct Monogenic Disorders; Considering Pros and Cons to CRISPR; Engineering Human iPSCs: ZFN, TALEN, CRISPR/Cas9 Advantages and Limitations; Sequencing and Quality Control Consideration.
Introduction to Genome Editing Technologies; ZFN and CRISPR/Cas Design Considerations; Nucleofection; Gene Knock Out Using Targeted Nucleases; Integration and Detection; Donor DNA Design and Nuclease Compatibility; Exercises for ZFN and CRISPR Design; Bioinformatics for Genome Editing Applications; Development of Genetically Modified Cell Lines Using K562 Cells (will allow attendees to run their own experiments and analyze their own results by the end of this workshop); Engineering iPSCs using CRISPR/Cas9 & HR Targeting Vectors; Introduction to Genome Editing using the CRISPR/Cas9 System; Review gRNA Design Principles, Cloning into SBI’s Cas9 SmartNuclease System, and Best Practices for Use of the CRISPR/Cas9 System for Genome Editing; HR Targeting Vectors for Genome Editing in iPSCs. This Could Cover Basic Knock-out and Gene Tagging Strategies Using SBI’s Existing PrecisionX HR Donors, and Could Also Include Use of Our PiggyBac-HR Vector (PBHR Donor) for Single Base Modifications in Exons, While Incorporating the Cas9/gRNA Constructs Built in the First Section; Generating Stable Isogenic Overexpression & Reporter Lines using the PinPoint Targeted Integration System; Generating PinPoint Platform iPS Cell Lines using the PhiC31 Integrase vs. Safe Harbor-Specific TALEN/HR Donor Combination; Generating Stable Isogenic Overexpression & Reporter Cell Lines using the PinPoint Targeting Integration system.
Class size will be limited to 24
Contains all notes, protocols, and reference material needed. No additional books are required.
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits. For a 3-day workshop, a participant receives 2.1 CEUs. For a 4-day workshop, a participant receives 2.8 CEUs. For a 5-day workshop, a participant receives 3.5 CEUs.
Related BioTech Courses
BioTech 18: Stem Cells
BioTech 47: Human Pluripotent Stem Cells (hiiPSC); Differentiation Neural Lineages
BioTech 49: Making iPSCs from Blood
BioTech 54: Making Cardiomyocytes from iPSCs
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop.
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.
1. All cancellations must be received in writing via email to Ms. Carline Coote at firstname.lastname@example.org.
2. Cancellations received after hours are time marked for the following day.
3. In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
4. All refund payments will be processed by the start of the initial workshop.
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility Reservations can be made online, see below.