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BioTech 92 | RNA Library Prep for Next Generation Sequencing (NGS) | 2-Day Hands On Workshop

August 14, 2018 to August 15, 2018

Registration occurs on a first-come, first-served basis. The deadline forregistration is one week before the first day of the course. If you are unable toregister before the deadline, please email: or call 301-496-7977for space availability.

NIH Only: Payment approval and authorization is done through your AO or labmanager. Receiving lab approval does not constitute enrollment. Fellows or those being sponsored by their lab can enroll using this form while waiting forauthorization of payment.

Workshops generally run from 9:00am - 5:00pm.

Register Now

Contract: SF-182

The training will also address:

  • Workflow and chemistry
  • QCs and troubleshooting
  • Sequencing data performance

Experts from academia and industry will teach how to prepare RNA-seq libraries Participants will prepare Total RNA sequencing libraries using the  NEB Next UltraII Directional RNA Library Prep Kit and  rRNA depletion kit (Human, Mouse and Rat) for Illumina next generation sequencing (NGS)* 

Control RNA will be provided.  Participants are welcome to bring one to Total RNA sample (Human, Mouse or Rat species). If bringing your own sample, please bring 5 ng – 1 ug total RNA (DNA-free) in up to 12 ul of Nuclease-free water, quantified by Qubit Fluorometer and quality checked by Bioanalyzer.  For questions regarding samples, please contact 

Participants should be comfortable with basic computer skills.

Class size is limited to 20 

Other FAES@NIH workshops relevant to RNAseq/NGS

BioTech 45 | Bioinformatics Analysis of Next Generation Sequencing Data

BioTech 74 | Advanced Transcriptomics (RNA Seq) Analysis

BioTech 75 | Metagenomics Data Analysis

(*This workshop is purely educational/training. FAES@NIH does not endorse any specific company/product; nor do not have any financial agreements/commission from NEB)

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours.   One CEU is equal to ten contact hours.   Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Refund Policy                                                                                                             
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 

50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 

0% refund, 100% full workshop credit is available for registrations cancelled 
3 days or less prior to the start of the workshop.  

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after hours are time marked for the following day.  
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation
A special rate is available to all BioTech Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility   Reservations can be made online, see below. 

DoubleTree by Hilton Bethesda - Washington DC


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