The Biophysical Methods for Protein Interaction Bootcamp will review strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR) and fluorescence spectroscopy for the characterization of biological macromolecules in solution.
The list of topics include:
- Experiment design in AUC: choice of concentrations, buffers, optical systems; sample volumes, noise types, meniscus considerations
- Conducting the AUC experiment: assembly of ultracentrifugal cells, adjustment of optics, operating the XLA/I
- Operating the ITC and experimental design
- Operating the DLS and experimental design.