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BIOL 003 | Methods in Molecular Biology

November 19, 2019 to November 22, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: registrar@faes.org or call 301-496-7977 for space availability. 

NIH employees paying with the signed vendor copy of the SF-182 form, please email:  registrar@faes.org

Register Now

Contract: SF-182

Workshops generally run from 9:00am - 5:00pm.​

This four day lecture and hands-on laboratory course is designed to provide the novice with an introduction to the molecular biology and related laboratory procedures which are widely used in biotechnology. The participants in this workshop will acquire skill in the use of "generic" methods, and through an understanding of such methods, improve their effectiveness as workers in a contemporary research laboratory.

Lecture Topics
Basics of mammalian & bacterial cell culture and cell maintenance. Types of cell lines, differences between cell line and primary culture; Introduction to Nucleic Acids; DNA and RNA extraction and Purification. Methods to quantify and analyze DNA and RNA.  Protein Purification & Analysis; Basics of genetic engineering: Restriction Enzymes and Restriction Mapping; Other Pertinent Enzymes; Cloning expression vectors; Principles, Theory, and Optimization of Polymerase Chain Reaction and its variations

Laboratory Topics
Lab basics: Pipetting; Cell culture techniques; primer design, mapping and molecular cloning; restriction enzyme digestion of recombinant plasmid and ligation; Nucleic Acids isolation, purification, and quantification; bacterial transformation; PCR; SDS-PAGE and Western blot; Bioinformatics tools

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Although no grades are given for Workshops, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% Refund or 100% Full Workshop Credit Available
Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available
Written Notificaiton: 4 to 13 days prior to start of program.
 0% Refund, 100% Full Workshop Credit Available
Written Notificaiton: 3 days or less prior to start of program.
 
Notification
All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov 
Cancellations received after hours are time marked for the following day.
In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation
A special rate is available to all FAES Workshop attendees. This includes complimentary continental breakfast, guest room Wi-Fi and discounted $15 parking per night. Hotel is within walking distance of the training facility.   Reservations can be made online, see below.

DoubleTree by Hilton Bethesda - Washington DC

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