Proximity Ligation Assay (PLA) provides the ability to see and quantitate protein posttranslational modifications, define complex interaction, illuminate complex protein clusters or amplify low abundant single events. Duolink is a versatile tool for detection, quantification and localization of cytoplasmic signaling events. This 2 day course will introduce participants to the key concepts of Proximity Ligation Assay and Duolink including technology development, assay design, antibody validation, best practices, trouble shooting and future applications. This workshop features hands-on lab exercises designed to provide participants with working knowledge of PLA & Duolink. Training slots are limited to 20, Registrations are First Come- First Serve basis.
Key Note Speakers:
Dr. Ryan Young (NCI/NIH) and Dr. Tracy Adair-Kirk (Millipore-Sigma)
Lecture Highlights
• Intro and History of PLA and Duolink
• Duolink Assay Workflow
• Assay design, Antibody selection and Validation
• Best Practices and Troubleshooting
• Novel Applications of PLA
• Future Directions, Multiplexing, IVD
• Data Analysis
Laboratory Highlights
• Cell Fixation and Permeabilization
• Antibody Incubation and Washing
• Ligation and Rolling Circle DNA Amplification
• Assay Imaging
• Results Analysis