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BIOC 053 | Super Resolution Microscopy

March 23, 2021 to March 26, 2021


Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability.

NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. Payments need to be received within 7 business days from date of reservation or before the start of a workshop, whichever comes first. Seat reservations will be cancelled if payment is not received prior to workshop.

Super Resolution Microscopy represents a group of recently developed light microscopic techniques that are able to exceed diffraction-limited resolution (less than 250 nm). This course, which will be presented entirely in virtual format this year, will focus on three types of Super Resolution Microscopy -- Single Molecule Localization Microscopy (e.g. PALM and STORM), Stimulated Emission Depletion Microscopy (STED), and Structured Illumination Microscopy (SIM). In addition, students will be exposed to other cutting-edge microscopy (e.g. lattice light sheet microscopy and iPALM) developed at the Janelia/HHMI Research Campus through its Advanced Imaging Center (AIC). The course will also cover fluorescent proteins and dyes, as well as the application of deep learning in super resolution imaging.

The course is designed for cell biologists with prior experience in light microscopy who wish to add super resolution microscopy to their research program. Participants will acquire theoretical understanding of super resolution microscopy through lectures given by experts in the field, and practical knowledge through remote Q&A lab sessions on SR microscopes given by microscope vendors.

Lecture Topics 
Introduction to Super Resolution Microscopy; Perils and Pitfalls in Super-Resolution Microscopy; Theoretical Background of SIM, PALM/dSTORM, and STED Imaging; Advances in Fluorescent Protein and Organic Dye Technology; Applications of Super Resolution Microscopy using SIM and dSTORM; Challenges Associated with Obtaining Good SIM, STORM and STED Images; Potential Artifacts Common to Super Resolution Imaging; Lattice Light Sheet Microscopy; Image Denoising Based on Deep Learning.


Laboratory Topics
Imaging specialists from the various microscope manufacturers will demo their instruments and answer student questions using Zoom. Some of the microscopes that will be showcased include: Leica SP8-STED, Nikon N-STORM, Zeiss Elyra7 Lattice SIM, and Zeiss LSM 880-AiryScan. In addition, the Shroff Lab at NIH will showcase their Instant SIM and their Dual View Plane Illumination microscope.  

Related FAES Workshops
BIOC 035 | Imaging – From IF and FISH to Automated and Confocal Microscopy
BIOC 062 | Image Processing and Analysis

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Training Rate
Discounted Training Rates
$1,075.00 - NIH Community (Trainees, Employees, Contractors, Volunteers, etc.)
$1,195.00 - Academia, US Government, US Military

Technology Fee

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day. 
All refund payments will be processed by the start of the initial workshop.

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