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BIOF 045 | Next Generation Sequencing Data Analysis

March 22, 2021 to March 26, 2021



Registration occurs on a first-come, first-served basis. Programs are open to general public. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7975 for space availability. 

NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. FAES must receive payment within 7 business days after reserving a seat or 3 business days before the start of the workshop, which ever comes first. If payment is not received in this time frame, your reservation will be canceled.

Course Overview
This course will introduce the basics of next generation sequencing (NGS) data analysis. We will introduce NGS methods and platforms, bioinformatics tools and standards, and data analysis workflows, specifically for DNA-seq (variant analysis) and RNA-seq (transcriptome analysis). The lectures will introduce common software libraries and algorithms used in the bioinformatics community. During the hands-on training sessions, we will perform alignment and analysis of NGS data from scratch using standard software libraries and pipelines.


Participants will start with a general introduction to working in a linux environment and running command-line tools. We will learn the common file formats: fasta, fastq, SAM, BAM, VCF, GTF


Next, we will walk through:

a. DNA-Seq variant calling analysis

b. RNA-Seq analysis with downstream analysis in R

Finally, we will touch on data retrieval and best practices for making your analysis readable and reproducible.


Basic computing skills; a background in Linux and bash scripting is not required though preferred. Past experience with R is expected.

Computer (PC, Mac, or Linux) and internet access are required; Simultaneous access to two screens is highly recommended for best learning experience. Examples include one computer with two screens (most preferred), two laptops, one laptop and one tablet, etc. 

Learning Materials

All learning materials/links will be shared on the CANVAS site.

Training Rate

Discounted Training Rates
$1,255.00 - NIH Community (Trainees, Employees, Contractors, Volunteers, etc.)
$1,395.00 - Academia, US Government, US Military

Technology Fee

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours.   One CEU is equal to ten contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.
All refund payments will be processed by the start of the initial workshop.

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