January 25, 2021 to January 29, 2021
Registration occurs on a firstcome, firstserved basis. The deadline for registration is one week before the first day of the course. If you are unable to register before the deadline, please email: firstname.lastname@example.org or call 301-496-7977 for space availability.
NIH Fellows or NIH community members being sponsored by their lab and awaiting payment authorization can tentatively hold a seat using the “Reserve A Seat” option. FAES must receive payment within 7 business days after reserving a seat or 3 business days before the start of the workshop, which ever comes first. If payment is not received in this time frame, your reservation will be canceled.
Genome engineering provides the ability to manipulate gene expression in a variety of cell types and animal models. This course will provide an overview of the current major genome engineering platforms and cover CRISPR genome editing technology in depth.
The first part of the course will focus on the general principles of genome editing technology including design, choice of format, delivery, efficiency, specificity, clonal isolation, genotyping, and validation. The second part of the course will address different applications including genome editing in mice, zebrafish, and induced pluripotent stem (iPS) cells, disease modeling, generation of reporter lines, and high throughput approaches. We will discuss strategies to make CRISPR gene editing more efficient, flexible, and specific. We will explore recent advances in the CRISPR field including base editors and epigenome editing. We will also examine sequencing and quality control considerations for genome editing projects. Hands-on training on in-silico tools and carefully designed team projects will accompany the lecture material to provide practical training in design, assembly, transfection, and detection/evaluation steps of a typical genome editing workflow.
General Principles of Genome Engineering; DNA Repair Mechanisms; CRISPR-mediated Gene Modulation, Epigenome Editing, Targeted Integration, Genome Editing Workflow, Gene Correction in iPSCs; Targeted Mutagenesis in Zebrafish; CRISPR Genome Engineering in ES Cells and Embryos for Mouse Model Generation; Developing Reporter Lines; Sequencing and Quality Control Considerations.
Class size will be limited to 32
This workshop is taught by subject matter experts from the National Institutes of Health and industry (MilliporeSigma).*
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits. For a 3-day workshop, a participant receives 2.1 CEUs. For a 4-day workshop, a participant receives 2.8 CEUs. For a 5-day workshop, a participant receives 3.5 CEUs.
General Training Rate
Discounted Training Rates
$1,255.00 - NIH Community (Trainees, Employees, Contractors, Volunteers, etc.)
$1,395.00 - Academia, US Government, US Military
100% tuition refund for registrations canceled 14 or more days prior to the start of the workshop.
50% tuition refund for registrations canceled between 4 to 13 days prior to the start of the workshop.
No refund will be issued for registrations canceled 3 days or less prior to the start of the workshop.
All cancellations must be received in writing via email to Ms. Carline Coote at email@example.com.
Cancellations received after hours are time marked for the following day.
All refund payments will be processed by the start of the initial workshop.
*This workshop is purely educational/training. FAES does not endorse any specific company/product; nor do not have any financial agreements/commission from MilliporeSigma.