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BIOF 074 (Formerly BioTech 74) | Advanced Transcriptome (RNA Seq) Analysis: Hands-on Training

December 17, 2019 to December 20, 2019

Registration occurs on a first-come, first-served basis. The deadline for registration is one week before the first day of the course.  If you are unable to register before the deadline, please email: or call 301-496-7977 for space availability. 

NIH Fellows or NIH community members being sponsored by their lab can enroll tentatively, using the Contract: SF-182 option on the workshop’s registration site while waiting for authorization of payment. Enrollment is not guaranteed until payment is received.


JOIN US MARCH 9 - 12, 2020

Apart from performing the routine differential expression analysis using two different suite of tools, this hands-on training will help participants learn advanced RNA-Seq analysis techniques and tools for detecting snps, fusion genes, allele specific expressions, circular RNAs, viral/bacterial sequence identification, alternative polyadenylation and transcriptional regulatory network analysis.


  • Participants will use a Graphic User Interface based Linux Desktop environment, specially configured for advanced RNA-Seq analysis in the Amazon Cloud
  • Training provided by experienced active NIH researchers
  • Cookbook style bound manual for all exercises
  • Continuing Education Credits

Who should attend

  • Researchers and academics who are interested in exploring the RNA-Seq data for more than what is routinely done.
  • Clinicians who are interested in discovering novel biological regulatory mechanisms looking for potential diagnostic biomarkers and therapeutic uses.
  • Biomedical students who are interested in pursuing research on advanced transcriptomics and transcriptional regulatory mechanisms.
  • Scientists in program administration interested in learning and understanding the latest methods and concepts in transcriptome analysis

Participants should be comfortable with basic computer skills

Hands-on Skills/Tools taught:

Differential expression analysis:         HISAT2 tools
Differential expression analysis:         Trinity tools
Fusion gene analysis:                         STAR-Fusion
SNP detection:                                    GATK
Allele specific expression:                  WASP
Circular RNA identification:                circexplorer
Alternative polyadenylation:                DaPars
Viral integration:                                  VirTect           
Network analysis                                 Cytoscape

Workshops generally run from 9:00am - 5:00pm.

Registration Fee
$695.00 - NIH Trainees (Fellows, Postdocs, Postbacs, Grad students workng at any NIH campus)
$895.00 - NIH Community (Working, Appointed, or Assigned to NIH Campus)
$999 - Academia, US Goverment, US Military
$1,199.00 - General Rates - All others

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Refund Policy
100% tuition refund for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund for registrations cancelled between 4 to 13 days prior to the start of the workshop.
No refund will be issued for registrations cancelled 3 days or less prior to the start of the workshop.   

All cancellations must be received in writing via email to Ms. Carline Coote at
Cancellations received after hours are time marked for the following day. 
All refund payments will be processed by the start of the initial workshop.

Suggested Accommodation

A special rate is available to all workshop attendees. This includes complimentary buffet breakfast, guest room Wi-Fi, and discounted $15 parking per night. The hotel provides complimentary shuttle service to/from the NIH campus. The hotel is also within walking distance of the training facility. Reservations can be made online, see below. 

The Bethesdan by Hilton



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